labtype™ methodology Search Results


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Illumina Inc beadarray
Purified donor CD4 T cells were transferred into BDF1 mice as described for Figs. 7 & 8, and host spleens from B10.D2 CD4->F1 and DBA CD4->F1 mice (n=4/group) were profiled by whole-genome expression analysis at day 14 using Illumina <t>BeadArray</t> methodology (see Methods). Heat map of differentially expressed genes (red = higher, blue = lower expression) was generated by data output from GenomeStudio and analysis using GenePattern and Gene Set Enrichment Analysis.
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Results of HLA‐B27 Using  rSSO DNA Typing Method

Journal: Cytometry. Part B, Clinical Cytometry

Article Title: Human HLA‐B27 typing using the BD™ HLA‐B27 kit on the BD FACSVia™ system: A multicenter study

doi: 10.1002/cyto.b.21630

Figure Lengend Snippet: Results of HLA‐B27 Using rSSO DNA Typing Method

Article Snippet: Adjudication of HLA‐B27 discordant results was performed using the reverse sequence‐specific oligonucleotide (rSSO) DNA typing method (LABType® SSO, One Lambda).

Techniques:

Purified donor CD4 T cells were transferred into BDF1 mice as described for Figs. 7 & 8, and host spleens from B10.D2 CD4->F1 and DBA CD4->F1 mice (n=4/group) were profiled by whole-genome expression analysis at day 14 using Illumina BeadArray methodology (see Methods). Heat map of differentially expressed genes (red = higher, blue = lower expression) was generated by data output from GenomeStudio and analysis using GenePattern and Gene Set Enrichment Analysis.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Intrinsic differences in donor CD4 T cell IL-2 production influence severity of parent-into-F1 murine lupus by skewing the immune response either towards help for B cells and a sustained autoantibody response or towards help for CD8 T cells and a down regulatory Th1 response

doi: 10.4049/jimmunol.1402782

Figure Lengend Snippet: Purified donor CD4 T cells were transferred into BDF1 mice as described for Figs. 7 & 8, and host spleens from B10.D2 CD4->F1 and DBA CD4->F1 mice (n=4/group) were profiled by whole-genome expression analysis at day 14 using Illumina BeadArray methodology (see Methods). Heat map of differentially expressed genes (red = higher, blue = lower expression) was generated by data output from GenomeStudio and analysis using GenePattern and Gene Set Enrichment Analysis.

Article Snippet: To determine whether these two pathways exhibit distinct gene expression signatures, spleens from B10.D2 CD4→F1, DBA CD4→F1, and uninjected control mice were profiled by genome-wide gene expression analysis at day 14, using Illumina BeadArray methodology (see Methods).

Techniques: Purification, Expressing, Generated